Dr. Nichollas Scott’s research interests focus on the application of mass spectrometry-based methodologies to biological systems and the characterization of protein properties not self-evident in the polypeptide sequence itself. During his PhD, he focused on the analysis of bacterial protein glycosylation with specific interest in Campylobacter jejuni and developed workflows to selectively enrich as well as identify glycoproteins from bacterial systems.
In November 2011, Dr. Scott was awarded a National Health and Medical Research Council/Early Career Post-Doctoral Fellowship to undertake further training in the application and development of mass spectrometry techniques to study protein/ligand interactions. Dr. Scott joined the Foster laboratory in June 2012, where he has explored the use of protein correlation profiling (PCP) coupled to SILAC to investigate protein-protein interactions with specific interest in the further development of this technology to look at membrane protein complexes.
Scott NE, Nothaft H, Edwards AV, Labbate M, Djordjevic SP, Larsen MR, Szymanski CM, Cordwell SJ. Modification of the Campylobacter jejuni N-linked glycan by EptC protein-mediated addition of phosphoethanolamine. J Biol Chem. 2012 Aug 24;287(35):29384-96. doi: 10.1074/jbc.M112.380212. Epub 2012 Jul 2. (PubMed abstract)
Iwashkiw JA, Seper A, Weber BS, Scott NE, Vinogradov E, Stratilo C, Reiz B, Cordwell SJ, Whittal R, Schild S, Feldman MF. Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation. PLoS Pathog. 2012;8(6):e1002758. doi: 10.1371/journal.ppat.1002758. Epub 2012 Jun 7. (PubMed abstract)
Scott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Mol Cell Proteomics. 2011 Feb;10(2):M000031-MCP201. doi: 10.1074/mcp.M000031-MCP201. Epub 2010 Apr 1. (PubMed abstract)
Nothaft H, Scott NE, Vinogradov E, Liu X, Hu R, Beadle B, Fodor C, Miller WG, Li J, Cordwell SJ, Szymanski CM. Diversity in the protein N-glycosylation pathways within the Campylobacter genus. Mol Cell Proteomics. 2012 Nov;11(11):1203-19. doi: 10.1074/mcp.M112.021519. Epub 2012 Aug 2. (PubMed abstract)
Lees-Miller RG, Iwashkiw JA, Scott NE, Seper A, Vinogradov E, Schild S, Feldman MF. A common pathway for O-linked protein-glycosylation and synthesis of capsule in Acinetobacter baumannii. Mol Microbiol. 2013 Jun 20. doi: 10.1111/mmi.12300. [Epub ahead of print] (PubMed abstract)